Figure 1From: High toxicity and specificity of the saponin 3-GlcA-28-AraRhaxyl-medicagenate, from Medicago truncatula seeds, for Sitophilus oryzaeChromatograms of Medicago truncatula seed extract. A. Injection into a C18 column (250 × 25 mm, 5 μm, Phenomenex) on an Agilent 1200 HPLC apparatus. The flow was 3.5 mL.min-1. The gradient was H2O + 0.04%TFA (solvent A) / ACN +0.04% TFA (solvent B) 90/10 for 5 min, then 60% solvent B for 25 min. The elution was monitored using a diode array detector at 210 nm. B. The fraction purified in A was injected into the same column with an elution under isocratic conditions (solvent B 30%). Peaks containing entomotoxic activity are indicated.Back to article page