Figure 3
From: Mitotic slippage in non-cancer cells induced by a microtubule disruptor, disorazole C1
Microtubule filaments form around nuclei after DZ treatment. a) Electron microscopy analysis of the nuclei in untreated RPE-hTERT cells and RPE-hTERT cells treated with 10 nM of DZ for 38 hours. A white arrow indicates the microtubule-like structures around the fragmented nucleus in the blowout image. b) Immunofluorescence microscopy analysis of tubulin structures in untreated RPE-hTERT cells and RPE-hTERT cells treated with 10 nM of DZ, 100 nM of VBL, 1 μM of TXL for 38 hours. Arrows indicate the microtubule-like structures around the fragmented nucleus labeled by anti-tubulin antibodies. un: untreated.