Figure 5

p53 is stabilized after DZ treatment, but is not required for mitotic slippage. IR: ionizing radiation, commonly used for inducing p53 in p53 knockdown cells to detect the efficiency of the siRNA knockdown. a) Immunoblot analyses of p53 in RPE-hTERT cells after indicated treatment. Upper panel: cells were treated with 10 nM of DZ for indicated time. Lower panel: cells were treated with 10 nM of DZ, 100 nM of VBL or 1 μM of TXL for 38 hours. b) Left panel: immunoblot analysis of p53 in RPE-hTERT p53-wild type and p53-knockdown cells. Right panel: percentages of fragmented nuclei in RPE-hTERT p53-wild type and p53-knockdown cells treated with 10 nM of DZ for 38 hours. Graph shows the average of three independent experiments. c) Upper panel: immunoblot analysis of p53 in HFF-hTERT vector only and HFF-hTERT shp53 (stable-knockdown) cells. Lower panel: percentages of fragmented nuclei in HFF-hTERT vector only and HFF-hTERT shp53 cells treated with 10 nM of DZ, 100 nM of VBL or 1 μM of TXL for 38 hours. Results in graph are averages of three independent experiments.