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Table 1 LC-MS/MS SRM parameters and sensitivity details for NAD+ metabolites and related nucleosides and nucleotides

From: NAD+ metabolite levels as a function of vitamins and calorie restriction: evidence for different mechanisms of longevity

Metabolite

Parent mass

Product mass

Cone voltage

Collision Energy

RT (min)

LOQ (pmol)

R2

RSD (%)

Nam

123

80

35

20

3.68

0.45

0.996

7

NA

124

80

35

20

7.15

1.20

0.999

12

Cyt

244

112

35

10

4.52

0.10

0.995

10

Urd

245

113

90

10

4.56

1.20

0.978

10

NR

255

123

50

10

4.07

0.20

0.992

7

NAR

256

124

35

10

4.80

0.06

0.984

5

Ino

269

137

80

10

6.31

0.07

0.993

3

CMP

324

112

35

10

12.05

0.68

0.999

6

UMP

325

97

90

15

12.22

0.21

0.989

8

NMN

335

123

35

10

9.04

0.50

0.988

9

QA

166 (-)

122

35

10

12.34

  

9

NaMN

336

124

35

10

11.66

0.18

0.999

5

IMP

349

137

80

10

13.59

0.13

0.997

5

NAD+

664

542

35

18

8.69

0.17

0.996

8

NaAD

665

542

35

20

11.59

0.26

0.987

8

NADH

666

649

35

20

11.77

0.06

0.991

16

NADP

744

604

35

25

14.01

0.87

0.995

9

NADPH

746

729

35

20

16.18

0.17

0.994

8