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Table 2 Fold changes in gene expression in cells treated with triptolide (55.5 nM)+LPS (1 μg/ml) as compared to those treated with LPS (1 μg/ml) alone.

From: MyD88-dependent and independent pathways of Toll-Like Receptors are engaged in biological activity of Triptolide in ligand-stimulated macrophages

a) Downregulation of LPS- stimulated genes by triptolide.
Gene Accession No. Gene name Fold change in response to treatment
Symbol    LPS* LPS+triptolide
Ccl2 NM_011333 Chemokine (C-C motif) ligand 2 19.90 -11.98
Ccl22 NM_011331 Chemokine (C-C motif) ligand 12 692.18 -8.53
Ccl3 NM_011337 Chemokine (C-C motif) ligand 3 4.48 -3.82
Ccl5 NM_013653 Chemokine (C-C motif) ligand 5 22.86 -19.05
Ccl7 NM_013654 Chemokine (C-C motif) ligand 7 96.67 -19.73
Ccl9 NM_011338 Chemokine (C-C motif) ligand 9 77.98 -30.74
Cxcl10 NM_021274 Chemokine (C-X-C motif) ligand 10 113.38 -39.18
Cxcl11 NM_019494 Chemokine (C-X-C motif) ligand 11 41.50 -13.01
Il10 NM_010548 Interleukin 10 55.52 -47.24
Il10ra NM_008348 Interleukin 10 receptor, alpha 4.01 -3.21
Il13ra1 NM_133990 Interleukin 13 receptor, alpha 1 13.13 -11.17
Il18 NM_008360 Interleukin 18 17.33 -7.68
Il1a NM_010554 Interleukin 1 alpha 2360.70 -102.68
Il1b NM_008361 Interleukin 1 beta 1140.14 -55.02
Il1f6 NM_019450 Interleukin 1 family, member 6 51.09 -39.18
Il2rg NM_013563 Interleukin 2 receptor, gamma chain 15.73 -5.66
Itgam NM_008401 Integrin alpha M 7.09 -5.91
Itgb2 NM_008404 Integrin beta 2 3.82 -4.63
Spp1 NM_009263 Secreted phosphoprotein 1 5.60 -3.25
Tgfb1 NM_011577 Transforming growth factor, beta 1 4.27 -1161.68
Tnf NM_013693 Tumor necrosis factor 58.69 -45.32
Tnfrsf1b NM_011610 Tumor necrosis factor receptor superfamily, member 1b 90.82 -16.94
Bcl3 NM_033601 B-cell leukemia/lymphoma 3 20.51 -20.30
Crebbp NM_001025432 CREB binding protein 2.92 -4.33
Csf2 NM_009969 Colony stimulating factor 2 (granulocyte-macrophage) 116.81 -61.53
Csf3 NM_009971 Colony stimulating factor 3 (granulocyte) 13568.7 -355.41
Gja1 NM_010288 Gap junction membrane channel protein alpha 1 4.65 -6.20
Il6 NM_031168 Interleukin 6 674.65 -256.59
Nfkb1 NM_008689 Nuclear factor of kappa light chain gene enhancer in B-cells 1, p105 32.63 -3.37
Nfkbia NM_010907 Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha 18.87 -2.93
Rel NM_009044 Reticuloendotheliosis oncogene 114.40 -14.86
Ripk1 NM_009068 Receptor (TNFRSF)-interacting serine-threonine kinase 1 3.18 -7.80
Tlr1 NM_030682 Toll-like receptor 1 43.35 -18.55
Tlr4 NM_021297 Toll-like receptor 4 3.27 -5.40
Tnfaip3 NM_009397 Tumor necrosis factor, alpha-induced protein 3 56.41 -5.15
Cd14 NM_009841 CD14 antigen 4.01 -7.56
Cd86 NM_019388 CD86 antigen 4.24 -2.90
Ifnb1 NM_010510 Interferon beta 1, fibroblast 5.56 -7.15
Irf3 NM_016849 Interferon regulatory factor 3 3.02 -58.79
Ly86 NM_010745 Lymphocyte antigen 86 5.15 -8.21
Mapk8 NM_016700 Mitogen activated protein kinase 8 5.68 -11.06
Ptgs2 NM_011198 Prostaglandin-endoperoxide synthase 2 566.13 -51.89
b) Upregulation of LPS down regulated genes by triptolide.
Gene symbol Accession No. Gene Name Fold change in response to treatment
    LPS* LPS+triptolide
Smad3 NM_016769 MAD homolog 3 (Drosophila) -13.20 4.02
Mapk8ip3 NM_013931 Mitogen-activated protein kinase 8 interacting protein 3 -5.19 4.21
  1. * Fold changes for LPS treatments were determined by comparing to base level expressions in untreated cells
  2. Positive values (≥+3) indicate stimulation while negative values (≤-3) represent suppression of gene expression.