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Table 1 In vitro inhibitory activity of CID755673 and its analogs for PKD

From: Novel protein kinase D inhibitors cause potent arrest in prostate cancer cell growth and motility

IC 50 (nM)
Compound PKD1 PKD2 PKD3
CID755673 182 ± 27 (n = 5) 280 ± 1.8 (n = 3) 227 ± 24 (n = 3)
Kb-NB142-70 28.3 ± 2.3 (n = 3) 58.7 ± 4.2 (n = 3) 53.2 ± 3.5 (n = 3)
Kb-NB165-09 82.5 ± 4.6 (n = 4) 141.6 ± 7.4 (n = 3) 98.5 ± 15.3 (n = 3)
Kb-NB165-31 114.1 ± 23.9 (n = 3) 162.9 ± 20.5 (n = 3) 91.1 ± 17.2 (n = 3)
Kb-NB165-92 111.2 ± 6 (n = 3) 100.7 ± 10.9 (n = 3) 58.8 ± 7.3 (n = 3)
Kb-NB184-02 192.8 ± 27.4 (n = 3) 463.2 ± 38.2 (n = 4) 324.7 ± 39.0 (n = 3)
  1. IC50 were determined for CID755673 and its analogs against PKD1, -2, and -3 using radiometric kinase activity assays. Each IC50 was calculated as the mean ± S.E.M. of at least three independent experiments with triplicate determinations at each concentration in each experiment as described in "Methods." *n, number of independent experiments