Figure 2

Strain sensitivity to different translation-inhibitory drugs. Wild type (WT) or gene deletion mutant strains (yploo9cΔ, yil137cΔ, ypl183w-a Δ, ydr056cC Δ and yjr111cΔ) were serially diluted to 10^-3 to 10^-6 and spotted on solid medium with sub-inhibitory concentrations of cycloheximide, paromomycin, 3-AT, streptomycin and neomycin as indicated, or without drugs (control). The plates were incubated at 30°C for 1-2 days. Deletion of ypl009c confers increased sensitivity to cycloheximide; yil137c and ypl183w-a to 3-AT, ydr056c to streptomycin and neomycin, and yjr111c to streptomycin.