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Figure 7 | BMC Chemical Biology

Figure 7

From: Chemical modification of L-glutamine to alpha-amino glutarimide on autoclaving facilitates Agrobacterium infection of host and non-host plants: A new use of a known compound

Figure 7

PCR of genomic DNA from leaf callus showing amplification of 490 bp gus gene. (a) Lane P: plasmid, Lane M: 0'GeneRuler™ 100 bp plus DNA ladder (Fermentas, Life Sciences), Lanes CA-AV: transformed aloe (CA: in absence of GlA40 and AV: in presence of GlA40), Lanes CL and L: transformed lavender (CL: in absence of GlA40, and L: in presence of GlA40), Lane CR: transformants in absence of GlA40 (CR: wild rose); (b) Lane T-T1: transformed tobacco (T: in absence of GlA40, T1: in presence of GlA40), Lanes C1 to C6: untransformed controls (1: aloe, 2: lavender, 3: wild rose, 4: grass, 5: fern, 6: apple rootstock); (c) Lanes R, G, F, A: transformed calli in presence of GlA40 (R: wild rose, G: grass, F: fern, A: apple rootstock) P: plasmid and Lanes C7-C9: transformants in absence of GlA40 (7: apple rootstock, 8: fern, 9: grass) and (d) Lane P: Plasmid DNA, Lane 1: maize transformed in presence of GlA40; Lane 2: transformant in absence of GlA40.

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BMC Chemical Biology

ISSN: 1472-6769