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Table 2 GUS expression after 2 days of co-cultivation in leaf explants transformed using different A. tumefaciens population densities

From: Chemical modification of L-glutamine to alpha-amino glutarimide on autoclaving facilitates Agrobacterium infection of host and non-host plants: A new use of a known compound

S. No. Plant species A. tumefaciens population density (cfu ml-1) versus GUS expression in different plant species
   1 × 107 1 × 108 1 × 109 1 × 1010
   Cont* GlA20 5-oxo proline GlA40 Cont GlA20 5-oxo proline GlA40 Cont GlA20 5-oxo proline GlA40 Cont GlA20 5-oxo proline GlA40
1. Nicotiana tabacum (tobacco) ++ - - ++ ++ - - ++ +++++ - - +++++ - - - -
2. Podophyllum hexandrum (Indian may apple) - - - - - - - + - - - ++++ - - - -
3. Aloe vera (aloe) - - - - - - - + - - - ++ - - - ++
4. Lavendula officinalis (lavender) - - - - - - - + - - - +++ - - - -
5. Rosa sp.
(Wild rose) 		- - - - - - - - - - - +++ - - - -
6. Malus domestica (apple rootstock) - - - + - - - ++ - - - +++++ - - - -
7. Dendrocalamus asper (bamboo) - - - ++ - - - +++ - - - +++++ - - - ++++
8. Cynodon dactylon (grass) - - - - - - - + - - - ++++ - - - ++++
9. Zea mays (maize) - - - + - - - ++ - - - +++ - - - +++
10. Oryza sativa (rice) - - - - - - - +++ +++ - - ++++ +++ - - ++++
11. Aurocaria (gymnosperm) - - - - - - - - - - - ++++ - - - ++++
12. Dryopteris (fern, pteridophyte) - - - - - - - - - - - ++++ - - - ++++
  1. where Cont* = control; + = one to five tiny blue spots; ++ = one to two large blue spots; +++ = small or faint blue patches; ++++ = large blue patch covering almost quarter of the leaf surface; and +++++ = entire leaf showed distinct blue color
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BMC Chemical Biology

ISSN: 1472-6769