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Table 3 GUS expression in response to different pH during co-cultivation on MSC containing GlA40 after transformation of leaf explants of different plant species using 1 ×109 cfu ml-1 A. tumefaciens

From: Chemical modification of L-glutamine to alpha-amino glutarimide on autoclaving facilitates Agrobacterium infection of host and non-host plants: A new use of a known compound

S. No. Plant Species pH GUS Expression
    (+) GlA40 (-) GlA40
1. Nicotiana tabacum (tobacco) 5.2 +++++ (< 1 day) +++++ (< 1 day)
   5.6 +++++ (< 1 day) +++++ (< 1 day)
   5.9 +++++ (< 1 day) +++++ (< 1 day)
   7.0 - -
2. Podophyllum hexandrum (Indian may apple) 5.2 - -
   5.6 ++++ (2 days) -
   5.9 +++ (2 days) -
   7.0 - -
3. Aloe vera (aloe) 5.2 +++ (1 day) -
   5.6 +++ (1 day) -
   5.9 - -
   7.0 - -
4. Lavendula officinalis (lavender) 5.2 +++ (1 day) -
   5.6 ++++ (1 day) -
   5.9 +++ (1 day) -
   7.0 - -
5. Rosa sp. (Wild rose) 5.2 - -
   5.6 +++ (1 day) + (4 days)
   5.9 +++ (1 day) + (5 days)
   7.0 - -
6. Malus domestica (apple rootstock) 5.2 ++++ (2 days) + (3 days)
   5.6 ++++ (2 days) + (3 days)
   5.9 ++++ (2 days) + (3 days)
   7.0 - -
7. Dendrocalamus asper (bamboo) 5.2 + (2 days) + (6 days)
   5.6 +++ (1 day) + (3 days)
   5.9 ++ (1 day) + (4 days)
   7.0 - -
8. Cynodon dactylon
(grass)
5.2 - -
   5.6 ++ (1 day) + (5 days)
   5.9 ++ (1 day) + (3 days)
   7.0 - -
9. Zea mays (maize) 5.2 +++ (1 day) + (3 days)
   5.6 +++ (1 day) + (3 days)
   5.9 ++++ (1 day) + (5 days)
   7.0 - -
10. Oryza sativa (rice) 5.2 ++ (1 day) + (4 days)
   5.6 +++ (1 day) + (2 days)
   5.9 +++ (1 day) + (2 days)
   7.0 - -
11. Aurocaria (gymnosperm) 5.2 - -
   5.6 ++++ (3 days) -
   5.9 - -
   7.0 - -
12. Dryopteris (fern, pteridophyte) 5.2 ++ (1 day) + (7 days)
   5.6 +++ (1 day) + (3 days)
   5.9 - -
   7.0 - -
  1. where + = one to five tiny blue spots; ++ = one to two large blue spots; +++ = small or faint blue patches; ++++ = large blue patch covering almost quarter of the leaf surface; +++++ = entire leaf showed distinct blue color and - = no GUS expression (Values in parenthesis represent the duration of co-cultivation)