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Table 3 GUS expression in response to different pH during co-cultivation on MSC containing GlA40 after transformation of leaf explants of different plant species using 1 ×109 cfu ml-1 A. tumefaciens

From: Chemical modification of L-glutamine to alpha-amino glutarimide on autoclaving facilitates Agrobacterium infection of host and non-host plants: A new use of a known compound

S. No.

Plant Species

pH

GUS Expression

   

(+) GlA40

(-) GlA40

1.

Nicotiana tabacum (tobacco)

5.2

+++++ (< 1 day)

+++++ (< 1 day)

  

5.6

+++++ (< 1 day)

+++++ (< 1 day)

  

5.9

+++++ (< 1 day)

+++++ (< 1 day)

  

7.0

-

-

2.

Podophyllum hexandrum (Indian may apple)

5.2

-

-

  

5.6

++++ (2 days)

-

  

5.9

+++ (2 days)

-

  

7.0

-

-

3.

Aloe vera (aloe)

5.2

+++ (1 day)

-

  

5.6

+++ (1 day)

-

  

5.9

-

-

  

7.0

-

-

4.

Lavendula officinalis (lavender)

5.2

+++ (1 day)

-

  

5.6

++++ (1 day)

-

  

5.9

+++ (1 day)

-

  

7.0

-

-

5.

Rosa sp. (Wild rose)

5.2

-

-

  

5.6

+++ (1 day)

+ (4 days)

  

5.9

+++ (1 day)

+ (5 days)

  

7.0

-

-

6.

Malus domestica (apple rootstock)

5.2

++++ (2 days)

+ (3 days)

  

5.6

++++ (2 days)

+ (3 days)

  

5.9

++++ (2 days)

+ (3 days)

  

7.0

-

-

7.

Dendrocalamus asper (bamboo)

5.2

+ (2 days)

+ (6 days)

  

5.6

+++ (1 day)

+ (3 days)

  

5.9

++ (1 day)

+ (4 days)

  

7.0

-

-

8.

Cynodon dactylon

(grass)

5.2

-

-

  

5.6

++ (1 day)

+ (5 days)

  

5.9

++ (1 day)

+ (3 days)

  

7.0

-

-

9.

Zea mays (maize)

5.2

+++ (1 day)

+ (3 days)

  

5.6

+++ (1 day)

+ (3 days)

  

5.9

++++ (1 day)

+ (5 days)

  

7.0

-

-

10.

Oryza sativa (rice)

5.2

++ (1 day)

+ (4 days)

  

5.6

+++ (1 day)

+ (2 days)

  

5.9

+++ (1 day)

+ (2 days)

  

7.0

-

-

11.

Aurocaria (gymnosperm)

5.2

-

-

  

5.6

++++ (3 days)

-

  

5.9

-

-

  

7.0

-

-

12.

Dryopteris (fern, pteridophyte)

5.2

++ (1 day)

+ (7 days)

  

5.6

+++ (1 day)

+ (3 days)

  

5.9

-

-

  

7.0

-

-

  1. where + = one to five tiny blue spots; ++ = one to two large blue spots; +++ = small or faint blue patches; ++++ = large blue patch covering almost quarter of the leaf surface; +++++ = entire leaf showed distinct blue color and - = no GUS expression (Values in parenthesis represent the duration of co-cultivation)