Figure 2

Nef-induced activation of mHck-TA. Recombinant downregulated mHck-TA activity was assayed over a range of kinase amounts in the presence of a 10-fold molar excess of purified recombinant Nef-SF2 (A) or Nef-Consensus (B) using the Z'Lyte method (see Materials and Methods). C) Inhibition of mHck-TA by NaPP1 in the presence or absence of Nef. Recombinant downregulated mHck-TA activity was assayed either alone or in the presence of a 10-fold molar excess of purified recombinant Nef-SF2 or Nef-Consensus over the range of NaPP1 concentrations shown using the Z'Lyte method (see Materials and Methods). All assays were performed in quadruplicate and data are shown as mean ± S.D. Each experiment was repeated twice and produced comparable results in each case.