Table 1 Kinetic parameters for RNA/DNA unmodified hammerhead ribozyme derivatives.
From: Redesigned and chemically-modified hammerhead ribozymes with improved activity and serum stability
Ribozyme | S-30 | S-550 | ||||
|---|---|---|---|---|---|---|
|  | kmax (min-1) | "Kd" nM | kmax/"Kd" (min-1μM-1) | kmax (min-1) | "Kd" nM | kmax/"Kd" (min-1μM-1) |
Rz-12/12 | 0.8 ± 0.1 | 7 ± 12 | 114 ± 200 | 0.7 ± 0.1 | 2600 ± 900 | 0.3 ± .2 |
Mz-12/12 | 0.22 ± 0.08 | 31 ± 6 | 7 ± 4 | 0.12 ± 0.01 | 240 ± 50 | 0.5 ± .2 |
Mrz-8/8 | 0.56 ± 0.06 | 210 ± 80 | 2.7 ± 1.3 | 0.005 ± 0.001 | 2200 ± 500 | 0.002 ± .001 |
Mrz-12/12 | 4.2 ± 0.3 | 75 ± 30 | 56 ± 26 | 1.6 ± 0.1 | 120 ± 50 | 13 ± 6 |
Rz-6/12 | 9 ± 1 | 400 ± 200 | 22 ± 13 | 0.6 ± 0.1 | 2800 ± 900 | 0.2 ± .1 |