Figure 2
From: The effect of amino acid deletions and substitutions in the longest loop of GFP
Oligonucleotides used for preparation of sgGFP mutants as described in M&M. For clear visualization, codons subjected to combinatorial deletion are alternatively labeled in bold and underlined. Asterisks represent nucleotide deletions. N represents an equimolar mixture of the four nucleotides and S a mixture of G and C. Restriction sites are double-underlined.