Figure 3From: The effect of amino acid deletions and substitutions in the longest loop of GFPWestern blotting analysis of deleted mutants grown at two different temperatures. (A) Single amino acid deletions comprised in the region 129–142 of sgGFP and the double mutant sgGFP-ΔI129/ΔD130 grown at 37°C. (B) Some sgGFP mutants grown at 30°C. S and P represent the soluble and insoluble fraction of the cells, respectively. Fractions S and P were run on different gels. Lane 1: sgGFP wt, lane 2: sgGFP-ΔI129, lane 3: sgGFP-ΔD130, lane 4: sgGFP-ΔF131, lane 5: sgGFP-ΔK132, lane 6: sgGFP-ΔE133, lane 7: sgGFP-ΔD134, lane 8: sgGFP-ΔG135, lane 9: sgGFP-ΔN136, lane 10: sgGFP-ΔI137, lane 11: sgGFP-ΔL138, lane 12: sgGFP-ΔG139, lane 13: sgGFP-ΔH140, lane 14: sgGFP-ΔK141, lane 15: sgGFP-ΔL142 and lane 16: sgGFP-ΔI129/ΔD130.Back to article page