BMC Chemical Biology

Table 1 DNA sequence of non-fluorescent clones chosen randomly from the library generated with COBARDE

From: The effect of amino acid deletions and substitutions in the longest loop of GFP

Clone	sgGFP modified region														Codons deleted
	Ile 129	Asp 130	Phe 131	Lys 132	Glu 133	Asp 134	Gly 135	Asn 136	Ile 137	Leu 138	Gly 139	His 140	Lys 141	Leu 142
Wt															0
1	ATC		TTC				GGC	AAC							10
2	ATC	GAC	TTC	AAG	GAA	GAC	GGC	AAC	ATC	CTG	GGA	CAC		CTG	1
3	ATC	GAC	TTC					AAC			GGA	CAC	AAA	CTG	6
4	ATC		TTC	AAG				AAC	ATC	CTG	GGA	CAC		CTG	5
5	ATC	GAC	TTC	AAG	GAA	GAC	GGC	AAC		CTG					5
6	ATC							AAC	ATC		GGA	CAC		CTG	8
7	ATC		TTC			GAG	GGC							CTG	9
8	ATC	GAC				GAC		AAC		CTG					9
9	ATC	GAC		AAG	GAA	GAC	GGC	AAC			GGA		AAA	CTG	4
10	ATC	GAC	TTC	AAG				AAC		CTG	GGA			CTG	6
11	ATC	GAC			GAA	GAC			ATC						9
12	ATC	GAC				GAC	GGC		ATC	CTG	GGA				7
13	ATC	GAC	TTC	AAG	GAA	GAC	GGC	AAC				CAC		CTG	4
14	ATC	GAC		AAG	GAA		GGC	AAC	ATC	CTG		CAC	AAA	CTG	3
15	ATC	GAC		AAG	GAA	GAC				CTG					8
16	ATC	GAC			GAA	GAC	GGC		ATC			CAC		CTG	6
17	ATC	GAC		AAG	GAA	GAC		AAC							8
18	ATC	GAC						AAC					AAA	CTG	9
19	ATC	GAC	TTC			GAC	GGC		ATC	CTG	GGA		AAA	CTG	4
20	ATC	GAC	TTC	AAG		GAC								CTG	8
21	ATC	GAC	TTC		GAA	GAC	GGC	AAC			GGA	CAC	AAA	CTG	3
22	ATC	GAC	TTC					AAC		CTG			AAA	CTG	7
23	ATC			AAG		GAC				CTG					10
24	ATC		TTC	AAG			GGC	AAC							9
25	ATC		TTC		GAA		GGC		ATC	CTG				CTG	7
26	ATC	GAC	TTC		GAA	GAC	GGC	AAC			GGA	CAC	AAA	CTG	3
27	ATC	GAC	TTC		GAA		GGC			CTG					8
28	ATC	G	TTC		GAA		GGC	AAC		CTG		CAC		CTG	6
29	ATC	GAC		AAG			GGC	AAC	ATC		GGA			CT	6
30	ATC		T	AAG		GAC		AAC		CTG	GGA		AAA	CTG	5
31	AC	GAC							ATC	CTG	GGA	CAC	AAA	CTG	6
32	ATC					GA	GGC	AAC		CTG	GGA		AAA		7
33	ATC	GAC		AAG				AAC		CTG	GA				8
oligo	TAG	CTG	AAG	TTC	CTT	CTG	CCG	TTG	TAG	GAC	CCT	GTG	TTT	GAC

Empty spaces represent deleted codons. Last row contains the oligonucleotide segment that was subjected to mutagenesis. Dots represent single nucleotide deletions. Nucleotide insertions are shown in gray letters.

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