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Table 1 DNA sequence of non-fluorescent clones chosen randomly from the library generated with COBARDE

From: The effect of amino acid deletions and substitutions in the longest loop of GFP

Clone sgGFP modified region Codons deleted
  Ile 129 Asp 130 Phe 131 Lys 132 Glu 133 Asp 134 Gly 135 Asn 136 Ile 137 Leu 138 Gly 139 His 140 Lys 141 Leu 142  
Wt                0
1 ATC   TTC     GGC AAC        10
2 ATC GAC TTC AAG GAA GAC GGC AAC ATC CTG GGA CAC   CTG 1
3 ATC GAC TTC      AAC    GGA CAC AAA CTG 6
4 ATC   TTC AAG     AAC ATC CTG GGA CAC   CTG 5
5 ATC GAC TTC AAG GAA GAC GGC AAC   CTG      5
6 ATC        AAC ATC   GGA CAC   CTG 8
7 ATC   TTC    GAG GGC        CTG 9
8 ATC GAC     GAC   AAC   CTG      9
9 ATC GAC   AAG GAA GAC GGC AAC    GGA   AAA CTG 4
10 ATC GAC TTC AAG     AAC   CTG GGA    CTG 6
11 ATC GAC    GAA GAC    ATC       9
12 ATC GAC     GAC GGC   ATC CTG GGA     7
13 ATC GAC TTC AAG GAA GAC GGC AAC     CAC   CTG 4
14 ATC GAC   AAG GAA   GGC AAC ATC CTG   CAC AAA CTG 3
15 ATC GAC   AAG GAA GAC     CTG      8
16 ATC GAC    GAA GAC GGC   ATC    CAC   CTG 6
17 ATC GAC   AAG GAA GAC   AAC        8
18 ATC GAC       AAC      AAA CTG 9
19 ATC GAC TTC    GAC GGC   ATC CTG GGA   AAA CTG 4
20 ATC GAC TTC AAG   GAC         CTG 8
21 ATC GAC TTC   GAA GAC GGC AAC    GGA CAC AAA CTG 3
22 ATC GAC TTC      AAC   CTG    AAA CTG 7
23 ATC    AAG   GAC     CTG      10
24 ATC   TTC AAG    GGC AAC        9
25 ATC   TTC   GAA   GGC   ATC CTG     CTG 7
26 ATC GAC TTC   GAA GAC GGC AAC    GGA CAC AAA CTG 3
27 ATC GAC TTC   GAA   GGC    CTG      8
28 ATC G TTC   GAA   GGC AAC   CTG   CAC   CTG 6
29 ATC GAC   AAG    GGC AAC ATC   GGA    CT 6
30 ATC   T AAG   GAC   AAC   CTG GGA   AAA CTG 5
31 AC GAC        ATC CTG GGA CAC AAA CTG 6
32 ATC      GA GGC AAC   CTG GGA   AAA   7
33 ATC GAC   AAG     AAC   CTG GA     8
oligo TAG CTG AAG TTC CTT CTG CCG TTG TAG GAC CCT GTG TTT GAC  
  1. Empty spaces represent deleted codons. Last row contains the oligonucleotide segment that was subjected to mutagenesis. Dots represent single nucleotide deletions. Nucleotide insertions are shown in gray letters.
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BMC Chemical Biology

ISSN: 1472-6769