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Table 2 Analysis of fluorescent and non-fluorescent mutants carrying single amino acid substitutions

From: The effect of amino acid deletions and substitutions in the longest loop of GFP

WT aa AS Hydrophobic aa Hydrophilic aa Acid Basic Stop
   W F P M L I V A G N Q S T C Y D E R K H X
F131 B    1   1 1   2*    1   1    1    1 1  
K132 E 1      1   1 1         1 2    
E133 E    1   2   1 3 1 1   1 1    1   3    
D134 E   1          1 2 2      2    
G135 B      1 1* 1*   1    1*   1 1       
N136 E   1 4 1 1   4 4    1   2      1 1 1  
I137 B      4   2   1    1*     1 2 2    1
L138 B   1   2 2 1    1*      1     1    
G139 E    1*   2    1 1 1 2 2 1 1      2 1  
H140 E    1    1*     1      1    2 2   
K141 E    2* 1     1   1    1      1   1  
L142 B 1      1 1   2 1   1     1      
  1. Mutants displaying a green-fluorescent phenotype to the naked eye are shown in bold numbers. Mutants displaying a white phenotype are shown in italic numbers. Mutants displaying a pale green-fluorescent phenotype are shown in underlined numbers. Those mutants labeled with asterisk turned pale green-fluorescent after prolonged growing at room temperature. The number represents the number of times that each mutant was found. In this table, empty spaces represent wild-type amino acids. B means buried amino acid; E, solvent exposed amino acid. During screening of fluorescent mutants, seven clones corresponded to wt sgGFP generated with the mixtures of degenerated oligonucleotides.
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BMC Chemical Biology

ISSN: 1472-6769