WT aa | AS | Hydrophobic aa | Hydrophilic aa | Acid | Basic | Stop |
---|
| | W | F | P | M | L | I | V | A | G | N | Q | S | T | C | Y | D | E | R | K | H | X |
---|
F131 | B | | |
1
| |
1
|
1
| |
2*
| | |
1
| |
1
| | |
1
| | |
1
|
1
| |
K132 | E |
1
| | | | |
1
| |
1
|
1
| | | | | | | |
1
|
2
| | | |
E133 | E | | |
1
| |
2
| |
1
|
3
|
1
|
1
| |
1
|
1
| | |
1
| |
3
| | | |
D134 | E | |
1
| | | | | | | | |
1
|
2
|
2
| | | | |
2
| | | |
G135 | B | | | | |
1
|
1*
|
1*
| |
1
| | |
1*
| |
1
|
1
| | | | | | |
N136 | E | |
1
|
4
|
1
|
1
| |
4
|
4
| | |
1
| |
2
| | | | |
1
|
1
|
1
| |
I137 | B | | | | |
4
| |
2
| |
1
| | |
1*
| | | |
1
|
2
|
2
| | |
1
|
L138 | B | |
1
| |
2
|
2
|
1
| | |
1*
| | | | |
1
| | | | 1 | | | |
G139 | E | | |
1*
| |
2
| | |
1
|
1
|
1
|
2
|
2
|
1
|
1
| | | | |
2
|
1
| |
H140 | E | | |
1
| | |
1*
| | | |
1
| | | | |
1
| | |
2
|
2
| | |
K141 | E | | |
2*
|
1
| | | |
1
| |
1
| | |
1
| | | | |
1
| |
1
| |
L142 | B |
1
| | | | |
1
|
1
| |
2
|
1
| |
1
| | | |
1
| | | | | |
- Mutants displaying a green-fluorescent phenotype to the naked eye are shown in bold numbers. Mutants displaying a white phenotype are shown in italic numbers. Mutants displaying a pale green-fluorescent phenotype are shown in underlined numbers. Those mutants labeled with asterisk turned pale green-fluorescent after prolonged growing at room temperature. The number represents the number of times that each mutant was found. In this table, empty spaces represent wild-type amino acids. B means buried amino acid; E, solvent exposed amino acid. During screening of fluorescent mutants, seven clones corresponded to wt sgGFP generated with the mixtures of degenerated oligonucleotides.