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Figure 3 | BMC Chemical Biology

Figure 3

From: Enhancement of intracellular γ-tocopherol levels in cytokine-stimulated C3H 10T1/2 fibroblasts: relation to NO synthesis, isoprostane formation, and tocopherol oxidation

Figure 3

NO synthesis and its effect on isoprostane formation. Confluent cultures of C3H 10T1/2 cells were treated for seven days as indicated with a combination of 10 μM α-tocopherol and 10 μM γ-tocopherol or with 25 μL ethanol as a control in 35 mm culture dishes (5 ml total media volume per dish). The cell culture media was then changed and cultures were treated with PBS (control), IFN/LPS, and/or 10 μM PBIT as indicated ± tocopherols. After seven days, cell culture media was assayed for 8-epi-prostaglandin F2α (A) or nitrite (B) as described in Methods. Values reported represent the mean ± SEM (N = 9). * p < 0.01 relative to corresponding control (ANOVA analysis of log-transformed values). Tocopherol-treated isoprostane levels are significantly different for each treatment group in comparison to non-tocopherol-treated cells (p < 0.01). Nitrite values between tocopherol-treated and control cells were not significantly different.

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BMC Chemical Biology

ISSN: 1472-6769