Figure 4From: Enhancement of intracellular γ-tocopherol levels in cytokine-stimulated C3H 10T1/2 fibroblasts: relation to NO synthesis, isoprostane formation, and tocopherol oxidationFormation of 8-epi-prostaglandin F2α by exogenous treatment of C3H 10T1/2 cells with spermine nonoate. Confluent cultures of C3H 10T1/2 cells were treated with either IFN/LPS or PBS at the time of weekly media change. Aqueous spermine nonoate was then added to give the final indicated concentration. After one week, media samples were analyzed for 8-epi-prostaglandin F2α as described in the Methods section. Values are reported as pg/ml 8-epi-prostaglandin F2α ± SEM (n = 6).Back to article page