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Table 1 Percent recovery and the ratio of γ-/α-tocopherol in the media fraction and cellular fraction of cells treated with IFN/LPS and/or PBIT as described in Figure 1. Values represent the mean of 6 determinations ± SEM.

From: Enhancement of intracellular γ-tocopherol levels in cytokine-stimulated C3H 10T1/2 fibroblasts: relation to NO synthesis, isoprostane formation, and tocopherol oxidation

 

Tocopherol Recovery# (%)

Ratio (γ/α tocopherol)

Treatment

Media

Cells

Total

Media

Cells

Media vs Cells

*p Value

 

αT|γT

αT|γT

αT|γT

   

Control

72.8|73.5

2.79|3.26

75.6|76.8

1.01 ± 0.01

1.17 ± 0.04

0.002

IFN/LPS

58.5|60.4

2.83|4.43

61.3|64.8

1.03 ± 0.01

1.57** ± 0.01

< 0.0001

IFN/LPS + PBIT

63.1|62.7

5.22|7.45

68.3|70.2

1.00 ± 0.01

1.43** ± 0.02

< 0.0001

PBIT

76.5|78.4

4.10|5.24

80.6|83.6

1.02 ± 0.01

1.27 ± 0.02

< 0.0001

  1. * Student t-test for difference between media and cells.
  2. ** ANOVA for differences between treatment groups of log transformed values p < 0.01 relative to control cells.
  3. # Based on treatment of 100 nmoles of each tocopherol per culture dish