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Table 2 Enhanced formation of α-tocopheryl-quinone in medium and cells of IFN/LPS-treated C3H 10T1/2 cells. Confluent cultures of C3H 10T1/2 cells were pre-treated for seven days as indicated with either 10 μM α-tocopherol, 10 μM γ-tocopherol, or with 25 μL ethanol as a control. The cell culture media was then changed and cultures treated with either PBS (control) or IFN/LPS for seven days. To another group of dishes treated with either tocopherol for 7 days, 100 μM of the NO donor, SIN-1, was given to cells for 2 hours. Samples of the media and cells were harvested and assayed for tocopherols and tocopherol oxidation products. Data are expressed as means ± SEM (n = 3).

From: Enhancement of intracellular γ-tocopherol levels in cytokine-stimulated C3H 10T1/2 fibroblasts: relation to NO synthesis, isoprostane formation, and tocopherol oxidation

 

Tocopherol

Oxidant

Tocopherol nmoles/106 cells (% of Control)

Tocopheryl Quinone nmoles/106 cells (% of Control)

5-Nitro-γ-Tocopherol nmoles/106 cells

Cells

α-Tocopherol

Control

1.32 ± 0.06

0.056 ± 0.002

-

 

α-Tocopherol

IFN/LPS

1.42 ± 0.15 (+7.5%)

0.072 ± 0.005 (+28.6%)**

-

 

α-Tocopherol

SIN-1

1.38 ± 0.02 (+4.5%)

0.290 ± 0.008 (+418%)*

-

 

γ-Tocopherol

Control

1.49 ± 0.07

0.0067 ± 0.0008

nd

 

γ-Tocopherol

IFN/LPS

2.50 ± 0.12 (+67.8%)*

0.0093 ± 0.0004 (+38.8%)**

nd

 

γ-Tocopherol

SIN-1

1.50 ± 0.05 (+0.7 %)

0.01 ± 0.005 (+49.2%)**

nd

   

nmoles/dish

nmoles/dish

nmoles/dish

Media

α-Tocopherol

Control

47.8 ± 2.0

0.79 ± 0. 08

-

 

α-Tocopherol

IFN/LPS

35.6 ± 1.1 (-25.5%)**

1.66 ± 0.06 (+110%)*

-

 

α-Tocopherol

SIN-1

1.9 ± 0.2 (-96%)*

34.0 ± 1.1 (+4,203%)*

-

 

γ-Tocopherol

Control

50.6 ± 1.4

0.30 ± 0.1

nd

 

γ-Tocopherol

IFN/LPS

49.7 ± 1.3 (-1.8%)

0.28 ± 0.05 (-6.6%)

nd

 

γ-Tocopherol

SIN-1

1.6 ± 0.5 (-96.7%)*

1.50 ± 0.02 (+400%)*

11.9 ± 0.3*

  1. Significantly different from its corresponding control value * p < 0.01; ** p < 0.05 nd = not detected