Figure 1From: Identification of cellular pathways affected by Sortin2, a synthetic compound that affects protein targeting to the vacuole in Saccharomyces cerevisiaeEffects of Sortin2 treatments on S. cerevisiae. (A) Dot-blot secretion assay using monoclonal antibodies raised against CPY, alkaline phosphatase (ALP) and 3-phosphoglycerate kinase (PGK). (B) Yeast growth in presence of 1% DMSO (●), 2 μg/ml (4.7 μM) Sortin2 (◆) and 20 μg/ml (47 μM) Sortin2 (■). (C) Western blot using CPY antibody of cell fractions of yeast upon 1% DMSO (1), 2 μg/ml (4.7 μM) Sortin2 (2) and 20 μg/ml (47 μM) Sortin2 (3) treatment.Back to article page