Figure 3

Gene-drug interactions in different physiological windows. A) Venn diagram depicting the number of significant growth defects (LPI < 0, p < 0.001) within a gene-drug mini-array. B-C) Comparing the relative growth reducing effect (LEC) of bioactive compounds on yeast WT populations to the number of knockouts displaying significantly reduced (LPI < 0, p < 0.001) tolerance to a specific compound. B) Considering growth efficiency (r² = 0.37). C) Considering growth lag (r² = 0.16). D) Frequency of clustering of bioactive compounds with similar mode-of-action (see results and discussion section). Repeated (n = 10) K-mean clusterings, in groups (k = 10) was performed and frequency of co-occurrence indicated. E) Drug induced multimodal growth in tif3 Δ in cerulenin. Black circles = observed OD values, red circles = derivatives (slopes) of observed OD values, red line = smooth estimate, ${\tilde{f}}^{\prime }$, of the function that best fits the derivatives of the observed OD values, green circles = maxima in ${\tilde{f}}^{\prime }$. F) Number of knockouts for which a specific drug displays multimodality.