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Figure 5 | BMC Chemical Biology

Figure 5

From: Catalytic inhibition of topoisomerase II by a novel rationally designed ATP-competitive purine analogue

Figure 5

BRCA1 mutant HCC1937 cells display increased sensitivity to catalytic topoisomerase II inhibition by ICRF-193 and QAP 1. (A) Left panel shows the immunoprecipitation (IP) of BRCA1 from HCC1937 BRCA1 mutant and HCC1937 BRCA1 reconstituted cells using an antibody that recognizes an internal epitope. In the Western blot (IB) using an antibody directed towards BRCA1 carboxyl-terminus the protein is only detected in IPs from the reconstituted cell line. Right panel: Levels of topoisomerase II alpha and beta in nuclear extracts of HCC1937 BRCA1 mutant and HCC1937 BRCA1 reconstituted cells. Histone H3 was used as a loading control. (B-D) Assessment of relative proliferation and colony formation in representative clonogenic growth assays with HCC1937 BRCA1 mutant (grey bars) and HCC1937 BRCA1 reconstituted (white bars) cells following continuous treatment for 9 days with ICRF-193, QAP 1 and 17-AAG, respectively.

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BMC Chemical Biology

ISSN: 1472-6769