Figure 5
From: Chemical tools selectively target components of the PKA system
Improvements on cAMP analogs for the purification of all R-subunit isoforms. Comparison of the protein yield eluted from the agarose with cGMP (red bars) followed by cAMP (blue bars) indicated on the plot. Data were normalised to the total protein yield from both elution steps using Sp-8-AEA-cAMPS agarose as determined by BCA assay. The lower panel shows a SDS-PAGE of samples from each elution fraction (equal volumes of original fractions, cGMP elution red framed, cAMP elution blue framed). M: molecular weight marker (Page-Ruler Unstained Protein Ladder, Fermentas).