Figure 7
From: Chemical tools selectively target components of the PKA system
Affinity enrichment of components of the cAMP-pathway using agonist and antagonist agaroses. Proteins from pig brain tissue were enriched using agonist (Sp-8-AEA-cAMPS) and antagonist (Rp-8-AHDAA-cAMPS) agaroses and subsequently eluted with cAMP. Fractions were separated on a 12% SDS-PAGE. supernatant: soluble protein fraction of pig brain after lysis, filtration and centrifugation; agonist/antagonist: protein eluted from the agonist/antagonist agarose with 25 mM cAMP. C-subunits (42 kDa), R-subunits RI (50 kDa) and RII (52 kDa) subunits are indicated, each identified by MS (data not shown). M: molecular weight marker (Page-Ruler, unstained protein ladder, Fermentas).