Table 2 Fold changes in gene expression in cells treated with triptolide (55.5 nM)+LPS (1 μg/ml) as compared to those treated with LPS (1 μg/ml) alone.
a) Downregulation of LPS- stimulated genes by triptolide. | ||||
|---|---|---|---|---|
Gene | Accession No. | Gene name | Fold change in response to treatment | |
Symbol | LPS* | LPS+triptolide | ||
Ccl2 | NM_011333 | Chemokine (C-C motif) ligand 2 | 19.90 | -11.98 |
Ccl22 | NM_011331 | Chemokine (C-C motif) ligand 12 | 692.18 | -8.53 |
Ccl3 | NM_011337 | Chemokine (C-C motif) ligand 3 | 4.48 | -3.82 |
Ccl5 | NM_013653 | Chemokine (C-C motif) ligand 5 | 22.86 | -19.05 |
Ccl7 | NM_013654 | Chemokine (C-C motif) ligand 7 | 96.67 | -19.73 |
Ccl9 | NM_011338 | Chemokine (C-C motif) ligand 9 | 77.98 | -30.74 |
Cxcl10 | NM_021274 | Chemokine (C-X-C motif) ligand 10 | 113.38 | -39.18 |
Cxcl11 | NM_019494 | Chemokine (C-X-C motif) ligand 11 | 41.50 | -13.01 |
Il10 | NM_010548 | Interleukin 10 | 55.52 | -47.24 |
Il10ra | NM_008348 | Interleukin 10 receptor, alpha | 4.01 | -3.21 |
Il13ra1 | NM_133990 | Interleukin 13 receptor, alpha 1 | 13.13 | -11.17 |
Il18 | NM_008360 | Interleukin 18 | 17.33 | -7.68 |
Il1a | NM_010554 | Interleukin 1 alpha | 2360.70 | -102.68 |
Il1b | NM_008361 | Interleukin 1 beta | 1140.14 | -55.02 |
Il1f6 | NM_019450 | Interleukin 1 family, member 6 | 51.09 | -39.18 |
Il2rg | NM_013563 | Interleukin 2 receptor, gamma chain | 15.73 | -5.66 |
Itgam | NM_008401 | Integrin alpha M | 7.09 | -5.91 |
Itgb2 | NM_008404 | Integrin beta 2 | 3.82 | -4.63 |
Spp1 | NM_009263 | Secreted phosphoprotein 1 | 5.60 | -3.25 |
Tgfb1 | NM_011577 | Transforming growth factor, beta 1 | 4.27 | -1161.68 |
Tnf | NM_013693 | Tumor necrosis factor | 58.69 | -45.32 |
Tnfrsf1b | NM_011610 | Tumor necrosis factor receptor superfamily, member 1b | 90.82 | -16.94 |
Bcl3 | NM_033601 | B-cell leukemia/lymphoma 3 | 20.51 | -20.30 |
Crebbp | NM_001025432 | CREB binding protein | 2.92 | -4.33 |
Csf2 | NM_009969 | Colony stimulating factor 2 (granulocyte-macrophage) | 116.81 | -61.53 |
Csf3 | NM_009971 | Colony stimulating factor 3 (granulocyte) | 13568.7 | -355.41 |
Gja1 | NM_010288 | Gap junction membrane channel protein alpha 1 | 4.65 | -6.20 |
Il6 | NM_031168 | Interleukin 6 | 674.65 | -256.59 |
Nfkb1 | NM_008689 | Nuclear factor of kappa light chain gene enhancer in B-cells 1, p105 | 32.63 | -3.37 |
Nfkbia | NM_010907 | Nuclear factor of kappa light chain gene enhancer in B-cells inhibitor, alpha | 18.87 | -2.93 |
Rel | NM_009044 | Reticuloendotheliosis oncogene | 114.40 | -14.86 |
Ripk1 | NM_009068 | Receptor (TNFRSF)-interacting serine-threonine kinase 1 | 3.18 | -7.80 |
Tlr1 | NM_030682 | Toll-like receptor 1 | 43.35 | -18.55 |
Tlr4 | NM_021297 | Toll-like receptor 4 | 3.27 | -5.40 |
Tnfaip3 | NM_009397 | Tumor necrosis factor, alpha-induced protein 3 | 56.41 | -5.15 |
Cd14 | NM_009841 | CD14 antigen | 4.01 | -7.56 |
Cd86 | NM_019388 | CD86 antigen | 4.24 | -2.90 |
Ifnb1 | NM_010510 | Interferon beta 1, fibroblast | 5.56 | -7.15 |
Irf3 | NM_016849 | Interferon regulatory factor 3 | 3.02 | -58.79 |
Ly86 | NM_010745 | Lymphocyte antigen 86 | 5.15 | -8.21 |
Mapk8 | NM_016700 | Mitogen activated protein kinase 8 | 5.68 | -11.06 |
Ptgs2 | NM_011198 | Prostaglandin-endoperoxide synthase 2 | 566.13 | -51.89 |
b) Upregulation of LPS down regulated genes by triptolide. | ||||
Gene symbol | Accession No. | Gene Name | Fold change in response to treatment | |
LPS* | LPS+triptolide | |||
Smad3 | NM_016769 | MAD homolog 3 (Drosophila) | -13.20 | 4.02 |
Mapk8ip3 | NM_013931 | Mitogen-activated protein kinase 8 interacting protein 3 | -5.19 | 4.21 |