Figure 4
Analysis of chromosome segregation in HL-60 cells by chromosome spreading. HL-60 cells were synchronized to the G1/S boundary by aphidicolin treatment for 16 hours and then released into fresh medium. After 6 hours release, the cells were treated as indicated below for 2 hours prior to preparation of chromosome spreads. (A) Cells treated with DMSO vehicle and 1 mM caffeine. (B) Cells treated with 400 nM ICRF-193 and 1 mM caffeine. (C) Cells treated with 10 μM QAP 1 and 1 mM caffeine. The following mitotic stages are shown: Late G2 (A1, B1 and C1), early prophase (A2, B2 and C2), prophase (A3, B3 and C3), prometaphase (A4, B4 and C4), metaphase (A5, B5 and C5), anaphase (A6, A7, B6, C6 and C7), telophase (A8–10, B7–B10 and C8–C10). Note, that each image represents a different cell. Arrows depict cells with evidence of chromosome malsegregation, arrow head depicts anaphase bridging.