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Table 2 GeneChip analysis of upregulated genes after 25-HC treatment of Huh-7 cells and Huh-7 cells bearing HCV subgenomic replicons.a

From: Transcriptional profiling of the effects of 25-hydroxycholesterol on human hepatocyte metabolism and the antiviral state it conveys against the hepatitis C virus

 

Description

Name

Accession #

Fold change 25-HC-treated

Fold change 25-HC-treated HCV replicon

1

cytochrome P450-IIB

hIIB3

M29873.1

1.5

1.5

2

BTG family, member 2

BTG2

NM_006763.1

1.6

---

3

epithelial membrane protein 3

EMP3

NM_001425.1

1.6

---

4

gro-beta

GROB

M57731.1

1.6

---

5

S100 calcium-binding protein A6 (calcyclin)

S100A6

NM_014624.2

1.6

-1.6

6

KIAA0063 gene product

KIAA0063

NM_014876.1

1.7

---

7

acetyl-Coenzyme A carboxylase alpha

ACACA

BE855983

1.7

-2.9

8

HUMBT Chromosome 16p13.3 Exon Homo sapiens genomic clone h-80

 

L48784

1.7

-2.4

9

centrin, EF-hand protein, 2, clone MGC:12421

 

BC005334.1

1.8

2

10

intercellular adhesion molecule 1 (CD54), human rhinovirus receptor

ICAM1

AI608725

1.9

1.6

11

diubiquitin

UBD

NM_006398.1

1.9

1.5

12

cDNA DKFZp564P1263 (from clone DKFZp564P1263)

 

AL136653.1

1.9

3.4

13

H. sapiens hGDS mRNA for smg GDS.

 

X63465.1

1.9

2.1

14

actin related protein 23 complex, subunit 4

ARPC4

NM_005718.1

1.9

---

15

natural killer cell transcript 4

NK4

NM_004221.1

2

-1.6

16

ATX1 (antioxidant protein 1, yeast) homolog 1

ATOX1

NM_004045.1

2.2

---

17

Human DNA with a hepatitis B virus surface antigen (HBsAg) gene

 

M20919

2.7

1.6

  1. a Fold change was calculated from the average of two independent hybridizations on U133A Affymetrix GeneChip arrays compared with an average expression value from analogous hybridizations from control cells that were not treated with 25-HC. Fold change for 25-HC-treated column was determined from the ratio of expression 25-HC treated Huh-7 cells/naĂŻve Huh-7 cells and for the 25-HC-treated HCV replicon column from the ratio of expression values from (replicon + 25-HC)/replicon. Dashes (---) indicate genes whose fold-change in gene expression did not exceed 1.5-fold and hence were omitted from the analysis.