Transcriptional profiling of the effects of 25-hydroxycholesterol on human hepatocyte metabolism and the antiviral state it conveys against the hepatitis C virus

Table 4 Comparative gene expression analysis of 25-HC-treated and HCV replicon-harboring Huh-7 cells with the changes in gene expression during HCV infection in chimpanzees with different outcomes of infection.^a

		Fold Change in Gene Expression over Naïve Huh-7 cells				Fold Change in Gene Expression during the Initial Rise in Viremia^c
Name	Accession #	Naïve Huh-7 + 25-HC	Huh-7 + luc/NS3-3'/Δ5B	Huh-7 + luc/NS3-3'/5.1	Huh-7 + luc/NS3-3'/5.1 + 25-HC	Naïve Chimpanzee	Sustained Clearance (SC)	Transient Clearance (TC)	Persistence (PS)
PROX1	NM_002763.1	-1.7	-2.0	-2.0	-1.9	---	3.5	1.6	-2.3
INSIG1^b	BG292233	-2.2	---	---	-1.8	---	1.6	2.9	---
	BE300521	-2.8	---	---	-2.8	N/A	N/A	N/A	N/A
	NM_005542.1	-2.2	---	---	-1.8	N/A	N/A	N/A	N/A
NK4	NM_004221.1	2.0	4.6	2.9	1.9	---	2.4	2.7	---
UBD	NM_006398.1	1.9	1.6	1.7	2.5	---	7.2	42.7	---

^a Comparisons were made with data collected from reference [11] for transcriptional profiles of 4 chimpanzees with different outcomes of viremia. See reference [11] for the details of the experiments for transcriptional profiling. The transcriptional profiling data from reference [11] was simply mined for the relevant data presented in this table; ^b Multiple INSIG1 Accessions correspond to the three INSIG1 probes; ^c Initial Rise in Viremia indicates gene expression levels at timepoint 3 after infection over timepoint 1 just prior to infection for each chimpanzee. Dashes (---) indicate genes whose fold-change in gene expression did not exceed 1.5-fold and hence were omitted from the analysis. N/A indicates transcripts that were not assessed in the chimpanzee gene expression profiles.