Table 1 Sequence parameters
Protein feature | Number of features | Calculated with | Remarks and references |
|---|---|---|---|
Amino acid residue composition | 40 | In-house script | Numbers and percentages of 20 amino acid residues |
Numbers and percentages of positive residues, negative residues, all charged residues, net charges | 8 | In-house script | Â |
Sequence length | 1 | Â | Â |
Predicted isoelectric point | 1 | ProtParam | [37] |
Number of C/H/N/O/S atoms | 5 | ProtParam | [37] |
Instability index | 1 | ProtParam | Predicted based on dipeptide composition [37] |
Instability class | 1 | ProtParam | Proteins with predicted in vivo half life ≥ 40 hrs considered as stable [37] |
Aliphatic index | 1 | ProtParam | [37] |
Gravy hydropathy index | 1 | ProtParam | [37] |
Predicted percentage of sheet, helix and coil | 3 | psipred | [40] |
Predicted percentage of buried/exposed residues | 2 | Accpro | [47] |
Predicted disordered regions including length of the longest coil, length of all coils, percentage of the longest coil, percentage of all coils; and corresponding features for rem465 and hotloop | 12 | disembl | [24] |
Tripeptide features (5 * 5 * 5) | 125 | In-house script | Â |