- Research article
- Open Access
HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck
© Pene-Dumitrescu et al; licensee BioMed Central Ltd. 2012
- Received: 23 September 2011
- Accepted: 15 March 2012
- Published: 15 March 2012
Nef is an HIV-1 accessory protein essential for viral replication and AIDS progression. Nef interacts with a multitude of host cell signaling partners, including members of the Src kinase family. Nef preferentially activates Hck, a Src-family kinase (SFK) strongly expressed in macrophages and other HIV target cells, by binding to its regulatory SH3 domain. Recently, we identified a series of kinase inhibitors that preferentially inhibit Hck in the presence of Nef. These compounds also block Nef-dependent HIV replication, validating the Nef-SFK signaling pathway as an antiretroviral drug target. Our findings also suggested that by binding to the Hck SH3 domain, Nef indirectly affects the conformation of the kinase active site to favor inhibitor association.
To test this hypothesis, we engineered a "gatekeeper" mutant of Hck with enhanced sensitivity to the pyrazolopyrimidine tyrosine kinase inhibitor, NaPP1. We also modified the RT loop of the Hck SH3 domain to enhance interaction of the kinase with Nef. This modification stabilized Nef:Hck interaction in solution-based kinase assays, as a way to mimic the more stable association that likely occurs at cellular membranes. Introduction of the modified RT loop rendered Hck remarkably more sensitive to activation by Nef, and led to a significant decrease in the Km for ATP as well as enhanced inhibitor potency.
These observations suggest that stable interaction with Nef may induce Src-family kinase active site conformations amenable to selective inhibitor targeting.
- Surface Plasmon Resonance
- Isothermal Titration Calorimetry
- Immobilize Metal Affinity Chromatography
- Kinase Active Site
Nef is an HIV-1 accessory protein that facilitates virus infectivity, replication, and immune evasion [1–3]. In non-human primate models of AIDS, high-titer viral replication and development of AIDS-like disease requires an intact Nef gene . Long-term non-progressive HIV infection in humans is also associated with Nef-defective HIV isolates in some cases [5, 6]. Complementary in vivo studies have shown that directed expression of Nef alone to HIV target cells induces an AIDS-like syndrome in transgenic mice [7–9]. Taken together, these studies underscore the importance of HIV-1 Nef in AIDS pathogenesis.
Nef is not known to exhibit any intrinsic enzymatic activity. Instead, Nef interacts with multiple host cell signaling pathways to enhance HIV-1 replication and promote AIDS progression . Previous work from our group has identified members of the Src kinase family as direct Nef effectors [11–15]. This kinase family includes Hck, a Src-family member expressed in macrophages, which are a critical HIV target cell type and viral reservoir. Nef interacts with the Hck SH3 domain, leading to constitutive Hck activation that may contribute to macrophage survival, MHC-I downregulation and M-tropic HIV replication [11, 12, 14, 16–18]. Nef has also been shown to bind and activate the Src-family kinases Lyn and c-Src, which exhibit a broader expression pattern including other HIV target cell types . Thus, Nef-dependent activation of Src family kinases is likely to occur in most HIV-infected cells.
Hck shares a similar domain organization and structural architecture with other members of the Src kinase family [19–21]. Key structural features include an N-terminal unique domain with sites for lipid attachment that drive membrane association, followed by the regulatory SH3 and SH2 domains, an SH2-kinase linker, the kinase domain, and a C-terminal negative regulatory tail. Nef binds to the Hck SH3 domain through a bipartite mechanism revealed in structural analyses of Nef:SH3 complexes [22–25]. Nef:SH3 interaction is dependent in part on a highly conserved PxxPxR motif, which forms a polyproline type II helix typical of most SH3 ligands. In addition, the αA and αB helices of Nef form a hydrophobic pocket that interacts with an Ile residue in the RT loop of the SH3 domain. Nef binding displaces the SH3 domain from its negative regulatory position on the back of the kinase domain, leading to kinase activation. Interestingly, mutation of the Nef PxxPxR motif completely abolished development of the AIDS-like phenotype in Nef-transgenic mice . Furthermore, crossing Nef transgenic mice into a hck- null background increased the latency for AIDS-like disease onset and decreased mortality . These data provide strong evidence that Src-family kinase activation by Nef is important for AIDS pathogenesis, and identify this signaling pathway as a target for therapeutic intervention.
Recently, we developed a chemical library screening assay based on Nef-dependent activation of Hck in vitro . Using this assay, we identified a series of diphenylfuropyrimidine (DFP) analogs that preferentially inhibit Hck in the presence of Nef. These compounds also potently blocked HIV-1 replication in a Nef-dependent manner , validating inhibitors of Nef-SFK signaling as potential antiretroviral agents. Our observation that DFP-based kinase inhibitors selectively inhibit the Nef:Hck complex suggested that Nef binding to the Hck SH3 domain induces structural changes in the kinase domain that favor inhibitor association. In the present study, we developed a system to test this hypothesis directly using a "gatekeeper" mutant of Hck with engineered sensitivity to the pyrazolopyrimidine analog, NaPP1 [26, 27]. This mutation involves substitution of the gatekeeper threonine (Thr338; numbering as per c-Src crystal structure ) with a much smaller alanine residue (Hck-TA mutant), providing access for NaPP1 to the hydrophobic cavity adjacent to the ATP binding site. This combination of mutant kinase and NaPP1 results in a high degree of inhibitor selectivity and potency both in vitro and in cell-based assays . Because NaPP1 binds to the Hck-TA active site in a specific location, it serves as a chemical probe for conformational changes that may occur in response to Nef binding. In addition to the gatekeeper mutation, we modified the SH3 domain to enhance interaction with Nef [29, 30]. This modification enabled stable association of Hck with Nef in solution-based kinase assays, thus mimicking the stable association that is likely to occur between Hck and Nef at cellular membranes . Use of this modified form of Hck combined with the selective inhibitor enabled us to demonstrate that Nef binding results in changes in the Km for ATP as well as inhibitor potency. These observations support the idea that Nef binding induces a unique active conformation of the Hck active site that can be targeted with selective inhibitors.
Recombinant protein expression and purification
Nef-SF2 and Nef-Consensus for kinase assays were expressed in E. coli with hexahistidine tags and purified by immobilized metal affinity chromatography (IMAC) as described elsewhere . For SPR experiments, the same two Nef proteins were purified without N-terminal His tags by anion-exchange chromatography (HiPrep Q FF; GE Healthcare). Both the His-tagged and untagged Nef proteins were further purified by gel filtration chromatography on a HiLoad 26/60 Superdex 75 column (GE Healthcare) with 20 mM Tris-HCl, pH 8.3, 100 mM NaCl and 3 mM DTT as mobile phase.
A cDNA clone for mouse Hck (mHck) was modified at its gatekeeper position (Thr338) with alanine, the C-terminal tail was changed to the autoregulatory sequence, Tyr-Glu-Glu-Ile (YEEI), and the N-terminal unique domain was replaced with a hexahistidine tag as described previously for human Hck . The coding sequence for the High Affinity RT loop (HART) was then introduced by replacing the wild-type SH3 RT loop sequence EAIHRE with TSPFPW using site-directed mutagenesis . The presence of all changes was confirmed by DNA sequence analysis of the entire Hck open reading frame. The mHck-T338A-YEEI (mHck-TA) and the mHck-T338A-HART-YEEI (mHck-TA-HART) coding sequences were subcloned into the baculovirus transfer vector pVL1392 (BD Biosciences) and the resulting plasmids used to create high-titer recombinant baculoviruses in Sf9 insect cells using Baculogold DNA and the manufacturer's protocol (BD Biosciences). Recombinant mHck proteins were expressed in Sf9 insect cells and purified using a combination of ion-exchange and IMAC chromatography as described previously for human Hck . Following purification, the mHck proteins were dialyzed against 20 mM Tris-HCl, pH 8.3, containing 100 mM NaCl and 3 mM DTT. The purity and concentration of each recombinant protein were confirmed by SDS-PAGE, densitometry and electrospray mass spectrometry.
The sequences encoding the wild-type hHck SH3 domain, mHck SH3 domain, and mHck SH3-HART mutant were amplified by PCR and subcloned into the bacterial expression vector, pET-14b (mHck SH3s) or pET-21a (hHck SH3). All three SH3 proteins were expressed in E. coli BL21(DE3)pLysS in the presence of 0.4 mM IPTG for 4 h at room temperature. Cells were sonicated in lysis buffer (20 mM Tris-HCl, pH 8.3, 10% glycerol, and 5 mM 2-mercaptoethanol), and soluble SH3 proteins were purified from clarified cell lysates using a combination of anion-exchange (HiPrep Q FF; GE Healthcare), IMAC (HiTrap Chelating HP; GE Healthcare), and size-exclusion chromatography (HiLoad 26/60 Superdex 75; GE Healthcare). Purified SH3 proteins were stored in 20 mM Tris-HCl, pH 8.3, 100 mM NaCl and 3 mM DTT. SH3 protein purity and concentration were confirmed by SDS-PAGE, densitometry and mass spectrometry.
Hydrogen exchange mass spectrometry (HXMS)
HXMS was used to investigate Nef:SH3 interaction as described  with the following modifications. Recombinant Hck SH3 and Nef proteins were equilibrated together at 4°C for at least 110 min before the initiation of the labeling reaction. Starting reactions consisted of the SH3 (50 μM) and Nef (117 μM) proteins in 20 mM Tris-HCl, pH 8.3, 100 mM NaCl, and 3 mM DTT. Deuterium labeling was initiated by 15-fold dilution of the binding reaction into D2O labeling buffer (20 mM Tris, pD 8.3, 100 mM NaCl, 3 mM DTT). Labeled proteins were injected onto a POROS 20 R2 protein trap and desalted with 0.05% trifluroacetic acid (TFA) at a flow rate of 500 μL/min. The proteins were eluted into the mass spectrometer using a linear 15% to 75% (v/v) acetonitrile gradient over 4 min at 50 μL/min with a Shimadzu HPLC system (LC-20AD). HPLC was performed using protiated solvents which results in the removal of deuterium from the side-chains and the amino/carboxy termini which exchange faster than backbone amide hydrogen atoms [32, 33]. Mass spectral analyses were carried out with a Waters LCT-PremierXE mass spectrometer with a standard electrospray source, a capillary voltage of 3.2 kV and a cone voltage of 35 V. The deuterium levels were not corrected for back-exchange  and reflect relative changes across the protein samples. The isotope envelopes in bimodal patterns were fit with two Gaussian functions whose widths were estimated from a single binomial isotopic envelope before and after the appearance of the bimodal pattern. The unfolding rates for those SH3 domains that presented evidence of a bimodal isotopic envelope were determined from the slope of pseudo-first-order kinetic plots of the decrease in the relative intensity of the lower mass envelope with time [34–36].
In vitro kinase assay
Protein-tyrosine kinase assays were performed using the FRET-based Z'-Lyte kinase assay kit and Tyr-2 peptide substrate according to the manufacturer's instructions (Life Technologies) and our previous work [14, 15, 27, 37]. All assays were performed in quadruplicate in low-volume, non-binding 384-well plates (Corning). To determine the Km for ATP, time course experiments were run at fixed concentrations of substrate (1 μM) and kinase in the presence of an ATP concentration range from 0 to 500 μM. The Km for ATP was then calculated from a plot of ATP concentration against initial reaction velocity followed by non-linear regression analysis (GraphPad Prism). For inhibition experiments, the assay was first optimized to determine the amount of kinase or kinase plus Nef (1:10 molar ratio) necessary to phosphorylate ~50% of the Tyr-2 peptide substrate in the presence of an ATP concentration equal to twice the Km. Kinases were pre-incubated with NaPP1 in kinase assay buffer (50 mM Hepes, pH 7.5, 10 mM MgCl2, and 1 mM EGTA, 0.01% Brij-35) for 30 min, followed by incubation with ATP and Tyr-2 peptide for 1 h at room temperature. Development reagent was then added to the reaction for an additional 1 h at room temperature, followed by addition of the stop reagent. Fluorescence was assessed at an excitation wavelength of 400 nm; coumarin fluorescence and the fluorescein FRET signal were monitored at 445 and 520 nm, respectively. Reactions run in the absence of ATP served as the 0% phosphorylation control, whereas a stoichiometrically phosphorylated Tyr2 peptide was used as the 100% phosphorylation control. Raw fluorescence values were corrected for background, and reaction endpoints calculated as emission ratios of coumarin fluorescence divided by the fluorescein FRET signal. These ratios were normalized to the ratio obtained with the 100% phosphorylation control peptide. IC50 values were calculated using a sigmoidal curve fit using GraphPad Prism. Additional details of the Z'-Lyte assay principle and calculation of percent inhibition has been described elsewhere [15, 27].
Surface plasmon resonance (SPR)
Evaluation of Nef binding to wild-type and HART SH3 domains by surface plasmon resonance (SPR).
ka (M-1s-1) × 105
kd (s-1) × 10-2
Nef concentrations (μM)
Human Hck SH3
1.60 ± 0.06
1.18 ± 0.04
0.07 ± 0.01
0, 0.007, 0.02, 0.062, 0.19, 0.56, 1.67, 5.0
Mouse Hck SH3
5.54 ± 1.77
7.00 ± 0.36
0.13 ± 0.03
0, 0.007, 0.02, 0.062, 0.19, 0.56, 1.67
Mouse Hck HART
14.20 ± 1.40
1.43 ± 0.01
0.01 ± 0.00
0, 0.002, 0.007, 0.02, 0.062, 0.19
Human Hck SH3
10.00 ± 0.80
11.80 ± 0.20
0.12 ± 0.01
0, 0.02, 0.062, 0.19, 0.56, 1.67, 5.0
Mouse Hck SH3
2.41 ± 0.05
1.85 ± 0.11
0.08 ± 0.01
0, 0.007, 0.02, 0.062, 0.19, 0.56, 1.67
Mouse Hck HART
15.70 ± 0.20
3.24 ± 0.16
0.02 ± 0.00
0, 0.002, 0.007, 0.02, 0.062, 0.19, 0.56
Expression and characterization of a mouse Hck gatekeeper mutant
To illustrate this chemical genetic concept, we modeled the gatekeeper alanine substitution using previous X-ray crystal structures of near-full-length downregulated Hck with either the ATP analog AMP-PNP or the NaPP1 analog PP1 bound to the active site [20, 21]. As shown in Figure 1B, alanine substitution of the gatekeeper position does not appear to influence kinase interaction directly with ATP. In contrast, when NaPP1 is overlaid via the corresponding pyrazolopyrimidine of PP1, the more bulky naphthyl substituent of NaPP1 clashes with Thr338 (Figure 1C). This clash is likely to be relieved by alanine substitution at this position because of the much smaller side chain. That said, additional conformational changes in this region are likely to be required to accommodate NaPP1 (see legend to Figure 1). Nevertheless, this model provides a useful framework for understanding the relationship of the gatekeeper substitution to NaPP1 sensitivity. More importantly, these mHck-TA gatekeeper mutants enabled use of NaPP1 as a specific chemical probe for this region of the kinase domain active site. This specific kinase-inhibitor pair allowed us to ask whether HIV-1 Nef binding to the SH3 domain influences the structure of the kinase domain as described below.
Nef does not influence wild-type mHck-TA ATP binding or competitive inhibition in solution-based kinase assays
HIV-1 Nef binding influences the Hck active site.
Km, ATP (μM)
IC50, NaPP1 (nM)
12.1 ± 2.0
mHck + Nef-SF2
14.8 ± 1.4
62 ± 12.6
53.3 ± 8.2
mHck-TA + Nef-SF2
68 ± 10.7
47.4 ± 6.0
mHck-TA + Nef-Consensus
84 ± 3.5
37.6 ± 4.6
105.2 ± 29.0
mHck-HART + Nef-SF2
49.34 ± 5.8
181 ± 28.7
8.5 ± 0.7
mHck-TA-HART + Nef-SF2
69 ± 17.6
2.4 ± 0.3
mHck-TA-HART + Nef-Consensus
76 ± 23.1
3.2 ± 0.2
mHck SH3 domain RT-loop mutants display enhanced affinity for HIV Nef
The data presented in the preceding section show that in solution, activation of mHck-TA by Nef does not perturb the Km for ATP or inhibitor binding. However, these assay conditions do not reflect the situation in cells, where membrane association is likely to stabilize Nef binding to Hck. Indeed, persistent interaction of Nef and Hck in cells has been recently been demonstrated using FRET . In contrast, when combined in solution, the stability of the Nef:Hck complex is solely a function of their concentrations. Thus, our inability to identify changes in the Km for ATP or in the IC50 for NaPP1 following Nef-induced activation of mHck-TA may be due to dissociation of the Hck:Nef complex in solution.
Before introducing the HART SH3 variant into near full-length mHck-TA, we first examined its conformational dynamics and interactions with Nef using hydrogen exchange mass spectrometry (HXMS). Wild-type mHck SH3 and the HART variant were expressed in bacteria and purified to homogeneity. Each SH3 domain was then incubated with D2O for various periods of time, resulting in exchange of backbone amide hydrogens in the protein with deuterium in the solvent [33, 50]. Amide hydrogen bonding retards deuterium exchange and amide hydrogens with high accessibility to solvent exchange more rapidly with deuterium than those that are buried. The kinetics of exchange provides detailed information on protein fluctuations in solution. In addition, changes in the rate of protein unfolding or refolding can be correlated with the strength of ligand binding.
Introduction of SH3-HART sensitizes near full-length mHck to activation by Nef
The enhanced activation of Hck-HART by Nef most likely reflects the enhanced affinity of the modified SH3 domain for Nef. However, HART modification may also reduce SH3 affinity for the SH2-kinase linker, thus creating a partial destabilization of the downregulated conformation of Hck. To control for this possibility, we compared the basal activity of Hck-TA with and without the HART substitution in the absence of Nef. We observed that the specific activity of Hck-TA-HART was less than two-fold higher than that of Hck-TA, consistent with the idea that the enhanced sensitivity of Hck-HART to Nef is primarily due its increased affinity for Nef interaction (data not shown).
Stable interaction with Nef impacts the mHck active site
Data presented in the previous section demonstrate that substitution of the wild-type mHck SH3 domain with mSH3-HART dramatically enhances Nef binding affinity for the SH3 domain, resulting in enhanced activation of mHck in vitro. These observations demonstrate that mHck-HART forms more stable complexes with Nef than wild-type mHck, allowing us to return to the question of whether Nef binding to the SH3 domain influences the Hck active site. We first determined whether the Km of mHck-TA-HART for ATP was affected by the presence of Nef. As shown in Table 2, the presence of Nef significantly reduced the Km for ATP with mHck-HART, and this effect was remarkably consistent for both Nef variants tested (~2.5-fold reduction in each case). A similar Km effect of Nef-SF2 was observed with recombinant mHck-HART in the absence of the TA mutation (Table 2). We next investigated whether the presence of Nef also affected inhibition of the Hck-TA-HART kinase by NaPP1, which occupies a binding site adjacent to and overlapping with the ATP binding site (modeled in Figure 1B). Here again, Nef-SF2 increased the sensitivity of mHck-TA-HART to inhibition by NaPP1 by more than three-fold (Table 2). Nef-Consensus also increased the apparent potency of NaPP1, albeit to a lesser extent. Together with the effect on the Km for ATP, these data provide strong evidence that the presence of Nef influences the conformation of the Hck active site.
In the present study, we developed a modified form of mHck that enabled an assessment of the impact of Nef binding on the kinase active site under solution assay conditions. Two important modifications were involved. First, the kinase domain gatekeeper residue (Thr338) was replaced with alanine, enabling site-specific inhibition with the pyrazolopyrimidine, NaPP1. Second, the RT-loop of the SH3 domain was modified to enhance Nef binding, thus promoting stable interaction in solution as demonstrated by SPR and HXMS. Using this modified form of Hck, we showed that Nef caused a significant decrease in the Km for ATP as well as an increase in the apparent potency of the site-specific inhibitor, NaPP1. These findings provide evidence that Nef binding not only dislodges the SH3 domain from its regulatory position on the back of the kinase domain, but also influences the conformation of active site residues involved in ATP binding as well as inhibition by NaPP1. Because Nef interacts with and activates Hck through its SH3 domain, rather than through direct interaction with the kinase domain, our results suggest that Nef influences the conformation of active site though an allosteric mechanism. Nef-mediated activation of Hck may alter the kinetics of kinase activity as well as substrate selection. Data presented here also suggest that this novel form of Hck will enable future high-throughput chemical library screens for next-generation compounds with enhanced specificity for the Nef:kinase complex. While use of the modified form of Hck described here may simplify high-throughput screening of chemical libraries, future work must ultimately address the sensitivity of myristoylated, full-length Nef and Hck complexes to inhibitors in the context of biological membranes.
This work was supported by funding from the National Institutes of Health (AI057083 to TS; GM086507 to JRE; GM82251 and AI76121 to JIY) and the Pennsylvania Department of Health. This is contribution 987 from the Barnett Institute.
- Arora VK, Fredericksen BL, Garcia JV: Nef: agent of cell subversion. Microbes Infect. 2002, 4: 189-199. 10.1016/S1286-4579(01)01527-1.PubMedView ArticleGoogle Scholar
- Fackler OT, Baur AS: Live and let die: Nef functions beyond HIV replication. Immunity. 2002, 16: 493-497. 10.1016/S1074-7613(02)00307-2.PubMedView ArticleGoogle Scholar
- Geyer M, Fackler OT, Peterlin BM: Structure-function relationships in HIV-1 Nef. EMBO Rep. 2001, 2: 580-585. 10.1093/embo-reports/kve141.PubMedPubMed CentralView ArticleGoogle Scholar
- Kestler H, Ringler DJ, Mori K, Panicali DL, Sehgal PK, Daniel MD, Desrosiers RC: Importance of the nef gene for maintenance of high viral loads and for development of AIDS. Cell. 1991, 65: 651-662. 10.1016/0092-8674(91)90097-I.PubMedView ArticleGoogle Scholar
- Kirchhoff F, Greenough TC, Brettler DB, Sullivan JL, Desrosiers RC: Absence of intact nef sequences in a long-term survivor with nonprogressive HIV-1 infection. N Engl J Med. 1995, 332: 228-232. 10.1056/NEJM199501263320405.PubMedView ArticleGoogle Scholar
- Mariani R, Kirchhoff F, Greenough TC, Sullivan JL, Desrosiers RC, Skowronski J: High frequency of defective nef alleles in a long-term survivor with nonprogressive human immunodeficiency virus type 1 infection. J Virol. 1996, 70: 7752-7764.PubMedPubMed CentralGoogle Scholar
- Hanna Z, Kay DG, Rebai N, Guimond A, Jothy S, Jolicoeur P: Nef harbors a major determinant of pathogenicity for an AIDS-like disease induced by HIV-1 in transgenic mice. Cell. 1998, 95: 163-175. 10.1016/S0092-8674(00)81748-1.PubMedView ArticleGoogle Scholar
- Hanna Z, Weng X, Kay DG, Poudrier J, Lowell C, Jolicoeur P: The pathogenicity of human immunodeficiency virus (HIV) type 1 Nef in CD4C/HIV transgenic mice is abolished by mutation of its SH3-binding domain, and disease development is delayed in the absence of Hck. J Virol. 2001, 75: 9378-9392. 10.1128/JVI.75.19.9378-9392.2001.PubMedPubMed CentralView ArticleGoogle Scholar
- Hanna Z, Kay DG, Cool M, Jothy S, Rebai N, Jolicoeur P: Transgenic mice expressing human immunodeficiency virus type 1 in immune cells develop a severe AIDS-like disease. J Virol. 1998, 72: 121-132.PubMedPubMed CentralGoogle Scholar
- Herna RG, Saksela K: Interactions of HIV-1 NEF with cellular signal transducing proteins. Front Biosci. 2000, 5: D268-D283. 10.2741/Renkema.View ArticleGoogle Scholar
- Briggs SD, Sharkey M, Stevenson M, Smithgall TE: SH3-mediated Hck tyrosine kinase activation and fibroblast transformation by the Nef protein of HIV-1. J Biol Chem. 1997, 272: 17899-17902. 10.1074/jbc.272.29.17899.PubMedView ArticleGoogle Scholar
- Lerner EC, Smithgall TE: SH3-dependent stimulation of Src-family kinase autophosphorylation without tail release from the SH2 domain in vivo. Nat Struct Biol. 2002, 9: 365-369.PubMedGoogle Scholar
- Choi HJ, Smithgall TE: Conserved residues in the HIV-1 Nef hydrophobic pocket are essential for recruitment and activation of the Hck tyrosine kinase. J Mol Biol. 2004, 343: 1255-1268. 10.1016/j.jmb.2004.09.015.PubMedView ArticleGoogle Scholar
- Trible RP, Emert-Sedlak L, Smithgall TE: HIV-1 Nef selectively activates SRC family kinases HCK, LYN, and c-SRC through direct SH3 domain interaction. J Biol Chem. 2006, 281: 27029-27038. 10.1074/jbc.M601128200.PubMedPubMed CentralView ArticleGoogle Scholar
- Emert-Sedlak L, Kodama T, Lerner EC, Dai W, Foster C, Day BW, Lazo JS, Smithgall TE: Chemical library screens targeting an HIV-1 accessory factor/host cell kinase complex identify novel antiretroviral compounds. ACS Chem Biol. 2009, 4: 939-947. 10.1021/cb900195c.PubMedPubMed CentralView ArticleGoogle Scholar
- Moarefi I, LaFevre-Bernt M, Sicheri F, Huse M, Lee C-H, Kuriyan J, Miller WT: Activation of the Src-family tyrosine kinase Hck by SH3 domain displacement. Nature. 1997, 385: 650-653. 10.1038/385650a0.PubMedView ArticleGoogle Scholar
- Hung CH, Thomas L, Ruby CE, Atkins KM, Morris NP, Knight ZA, Scholz I, Barklis E, Weinberg AD, Shokat KM: HIV-1 Nef assembles a Src family kinase-ZAP-70/Syk-PI3K cascade to downregulate cell-surface MHC-I. Cell Host Microbe. 2007, 1: 121-133. 10.1016/j.chom.2007.03.004.PubMedView ArticleGoogle Scholar
- Komuro I, Yokota Y, Yasuda S, Iwamoto A, Kagawa KS: CSF-induced and HIV-1-mediated distinct regulation of Hck and C/EBPbeta represent a heterogeneous susceptibility of monocyte-derived macrophages to M-tropic HIV-1 infection. J Exp Med. 2003, 198: 443-453. 10.1084/jem.20022018.PubMedPubMed CentralView ArticleGoogle Scholar
- Engen JR, Wales TE, Hochrein JM, Meyn MA, Banu OS, Bahar I, Smithgall TE: Structure and dynamic regulation of Src-family kinases. Cell Mol Life Sci. 2008, 65: 3058-3073. 10.1007/s00018-008-8122-2.PubMedView ArticleGoogle Scholar
- Schindler T, Sicheri F, Pico A, Gazit A, Levitzki A, Kuriyan J: Crystal structure of Hck in complex with a Src family-selective tyrosine kinase inhibitor. Mol Cell. 1999, 3: 639-648. 10.1016/S1097-2765(00)80357-3.PubMedView ArticleGoogle Scholar
- Sicheri F, Moarefi I, Kuriyan J: Crystal structure of the Src family tyrosine kinase Hck. Nature. 1997, 385: 602-609. 10.1038/385602a0.PubMedView ArticleGoogle Scholar
- Lee C-H, Saksela K, Mirza UA, Chait BT, Kuriyan J: Crystal structure of the conserved core of HIV-1 Nef complexed with a Src family SH3 domain. Cell. 1996, 85: 931-942. 10.1016/S0092-8674(00)81276-3.PubMedView ArticleGoogle Scholar
- Grzesiek S, Bax A, Clore GM, Gronenborn AM, Hu JS, Kaufman J, Palmer I, Stahl SJ, Wingfield PT: The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase. Nat Struct Biol. 1996, 3: 340-345. 10.1038/nsb0496-340.PubMedView ArticleGoogle Scholar
- Stangler T, Tran T, Hoffmann S, Schmidt H, Jonas E, Willbold D: Competitive displacement of full-length HIV-1 Nef from the Hck SH3 domain by a high-affinity artificial peptide. Biol Chem. 2007, 388: 611-615.PubMedView ArticleGoogle Scholar
- Jung J, Byeon IJ, Ahn J, Gronenborn AM: Structure, dynamics, and Hck interaction of full-length HIV-1 Nef. Proteins. 2011, 79: 1609-1622. 10.1002/prot.22986.PubMedPubMed CentralView ArticleGoogle Scholar
- Bishop AC, Shah K, Liu Y, Witucki L, Kung C, Shokat KM: Design of allele-specific inhibitors to probe protein kinase signaling. Curr Biol. 1998, 8: 257-266. 10.1016/S0960-9822(98)70198-8.PubMedView ArticleGoogle Scholar
- Pene-Dumitrescu T, Smithgall TE: Expression of a Src family kinase in chronic myelogenous leukemia cells induces resistance to imatinib in a kinase-dependent manner. J Biol Chem. 2010, 285: 21446-21457. 10.1074/jbc.M109.090043.PubMedPubMed CentralView ArticleGoogle Scholar
- Xu W, Doshi A, Lei M, Eck MJ, Harrison SC: Crystal structures of c-Src reveal features of its autoinhibitory mechanism. Mol Cell. 1999, 3: 629-638. 10.1016/S1097-2765(00)80356-1.PubMedView ArticleGoogle Scholar
- Horenkamp FA, Breuer S, Schulte A, Lulf S, Weyand M, Saksela K, Geyer M: Conformation of the dileucine-based sorting motif in HIV-1 Nef revealed by intermolecular domain assembly. Traffic. 2011, 12: 867-877. 10.1111/j.1600-0854.2011.01205.x.PubMedView ArticleGoogle Scholar
- Hiipakka M, Poikonen K, Saksela K: SH3 domains with high affinity and engineered ligand specificities targeted to HIV-1 Nef. J Mol Biol. 1999, 293: 1097-1106. 10.1006/jmbi.1999.3225.PubMedView ArticleGoogle Scholar
- Trible RP, Emert-Sedlak L, Wales TE, Ayyavoo V, Engen JR, Smithgall TE: Allosteric loss-of-function mutations in HIV-1 Nef from a long-term non-progressor. J Mol Biol. 2007, 374: 121-129. 10.1016/j.jmb.2007.09.009.PubMedPubMed CentralView ArticleGoogle Scholar
- Wales TE, Engen JR: Hydrogen exchange mass spectrometry for the analysis of protein dynamics. Mass Spectrom Rev. 2006, 25: 158-170. 10.1002/mas.20064.PubMedView ArticleGoogle Scholar
- Zhang Z, Smith DL: Determination of amide hydrogen exchange by mass spectrometry: a new tool for protein structure elucidation. Protein Sci. 1993, 2: 522-531.PubMedPubMed CentralView ArticleGoogle Scholar
- Engen JR, Smithgall TE, Gmeiner WH, Smith DL: Identification and localization of slow, natural cooperative unfolding in the Hck SH3 domain by amide hydrogen exchange and mass spectrometry. Biochemistry. 1997, 36: 14384-14391. 10.1021/bi971635m.PubMedView ArticleGoogle Scholar
- Gmeiner WH, Xu I, Horita DA, Smithgall TE, Engen JR, Smith DL, Byrd RA: Intramolecular binding of a proximal PPII helix to an SH3 domain in the fusion protein SH3Hck: PPIIhGAP. Cell Biochem Biophys. 2001, 35: 115-126. 10.1385/CBB:35:2:115.PubMedView ArticleGoogle Scholar
- Hochrein JM, Lerner EC, Schiavone AP, Smithgall TE, Engen JR: An examination of dynamics crosstalk between SH2 and SH3 domains by hydrogen/deuterium exchange and mass spectrometry. Protein Sci. 2006, 15: 65-73. 10.1110/ps.051782206.PubMedPubMed CentralView ArticleGoogle Scholar
- Pene-Dumitrescu T, Peterson LF, Donato NJ, Smithgall TE: An inhibitor-resistant mutant of Hck protects CML cells against the antiproliferative and apoptotic effects of the broad-spectrum Src family kinase inhibitor A-419259. Oncogene. 2008, 27: 7055-7069. 10.1038/onc.2008.330.PubMedPubMed CentralView ArticleGoogle Scholar
- Murphy M, Jason-Moller L, Bruno J: Using Biacore to measure the binding kinetics of an antibody-antigen interaction. Curr Protoc Protein Sci. 2006, Chapter 19:Google Scholar
- Jason-Moller L, Murphy M, Bruno J: Overview of Biacore systems and their applications. Curr Protoc Protein Sci. 2006, Chapter 19:Google Scholar
- Shugars DC, Smith MS, Glueck DH, Nantermet PV, Seillier-Moiseiwitsch F, Swanstrom R: Analysis of human immunodeficiency virus type 1 nef gene sequences present in vivo. J Virol. 1993, 67: 4639-4650.PubMedPubMed CentralGoogle Scholar
- Bishop AC, Buzko O, Shokat KM: Magic bullets for protein kinases. Trends Cell Biol. 2001, 11: 167-172. 10.1016/S0962-8924(01)01928-6.PubMedView ArticleGoogle Scholar
- Bishop AC, Ubersax JA, Petsch DT, Matheos DP, Gray NS, Blethrow J, Shimizu E, Tsien JZ, Schultz PG, Rose MD: A chemical switch for inhibitor-sensitive alleles of any protein kinase. Nature. 2000, 407: 395-401. 10.1038/35030148.PubMedView ArticleGoogle Scholar
- Liu Y, Bishop A, Witucki L, Kraybill B, Shimizu E, Tsien J, Ubersax J, Blethrow J, Morgan DO, Shokat KM: Structural basis for selective inhibition of Src family kinases by PP1. Chem Biol. 1999, 6: 671-678. 10.1016/S1074-5521(99)80118-5.PubMedView ArticleGoogle Scholar
- Fan QW, Zhang C, Shokat KM, Weiss WA: Chemical genetic blockade of transformation reveals dependence on aberrant oncogenic signaling. Curr Biol. 2002, 12: 1386-1394. 10.1016/S0960-9822(02)01070-9.PubMedView ArticleGoogle Scholar
- Wong S, McLaughlin J, Cheng D, Zhang C, Shokat KM, Witte ON: Sole BCR-ABL inhibition is insufficient to eliminate all myeloproliferative disorder cell populations. Proc Natl Acad Sci USA. 2004, 101: 17456-17461. 10.1073/pnas.0407061101.PubMedPubMed CentralView ArticleGoogle Scholar
- Porter M, Schindler T, Kuriyan J, Miller WT: Reciprocal regulation of Hck activity by phosphorylation of Tyr(527) and Tyr(416). Effect of introducing a high affinity intramolecular SH2 ligand. J Biol Chem. 2000, 275: 2721-2726. 10.1074/jbc.275.4.2721.PubMedView ArticleGoogle Scholar
- Saksela K, Cheng G, Baltimore D: Proline-rich (PxxP) motifs in HIV-1 Nef bind to SH3 domains of a subset of Src kinases and are required for the enhanced growth of Nef+ viruses but not for down-regulation of CD4. EMBO J. 1995, 14: 484-491.PubMedPubMed CentralGoogle Scholar
- Arold S, O'Brien R, Franken P, Strub MP, Hoh F, Dumas C, Ladbury JE: RT loop flexibility enhances the specificity of Src family SH3 domains for HIV-1 Nef. Biochemistry. 1998, 37: 14683-14691. 10.1021/bi980989q.PubMedView ArticleGoogle Scholar
- Lee C-H, Leung B, Lemmon MA, Zheng J, Cowburn D, Kuriyan J, Saksela K: A single amino acid in the SH3 domain of Hck determines its high affinity and specificity in binding to HIV-1 Nef protein. EMBO J. 1995, 14: 5006-5015.PubMedPubMed CentralGoogle Scholar
- Englander SW, Kallenbach NR: Hydrogen exchange and structural dynamics of proteins and nucleic acids. Q Rev Biophys. 1983, 16: 521-655. 10.1017/S0033583500005217.PubMedView ArticleGoogle Scholar
- Hochrein JM, Wales TE, Lerner EC, Schiavone AP, Smithgall TE, Engen JR: Conformational features of the full-length HIV and SIV Nef proteins determined by mass spectrometry. Biochemistry. 2006, 45: 7733-7739. 10.1021/bi060438x.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.